CHEMILUMINESCENT DETECTION OF THYMINE HYDROPEROXIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Overview

Title: 

CHEMILUMINESCENT DETECTION OF THYMINE HYDROPEROXIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Abstract: 

High-performance liquid chromatography (HPLC) with chemiluminescence detection was used to detect thymine hydroperoxides, which are expected to occur in biological tissues as primary products in free radical-mediated DNA damage. The thymine hydroperoxides were chemically prepared by the acid-catalysed hydrogen peroxide oxidation of thymine and 5-hydroxymethyluracil and their hydroperoxide products were identified as trans- or cis-5-chloro-6-hydroperoxy-5,6-dihydrothymine and 5-hydroperoxymethyluracil, respectively, by means of NMR, IR and mass spectrometry. The results confirmed that these thymine hydroperoxides art specifically detectable at picomole levels by the described HPLC method with high reproducibility.

Authors: 

Rie Saeki, Humio Inaba, Toshihide Suzuki, Teruo Miyazawa

DOI: 

Journal: 

Journal Of Chromatography

Year: 

1992

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